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KMID : 1143620170210010039
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Korean Journal of Nuclear Medicine Technology
2017 Volume.21 No. 1 p.39 ~ p.43
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Comparison of Results According to Reaction Conditions of Thyroglobulin Test
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Joung Seung-Hee
Lee Young-Ji
Moon Hyung-Ho
Yoo So-Yoen
Kim Nyun-Ok
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Abstract
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Purpose : Thyroglobulin (Tg) is a biologic marker of differentiated thyroid carcinoma (DTC), produced by normal thyroid tissue or thyroid cancer tissue. Therefore, the Tg values of DTC patients is the most specific indicator for judging whether recurrence occur or whether the remaining thyroid cancer is present. Thyroid cancer is currently the most common cancer in Korea, of which 90% is differentiated thyroid cancer. The number of patients with thyroid disease of this application also increased, and an accurate and prompt results are required. However, the incubation time of the Tg commonly takes about 24 hours in our hospital, and the result reporting time is delayed, and We could not satisfied with the requirements of clinical departments and patients. In order to fulfill these requirements, experiments were conducted by shortening the incubation time between company B’s Kit currently in use and company C’s Kit used in other hospitals. Through these experiments, we could perform the correlation with the original method and shortening method, and could find the optimum reaction time to satisfy the needs of the departments and the patients, and we will improve the competitiveness with the EIA examination.
Materials and Methods : In September 2016, we tested 65 patients company B's kit and company C's kit by three incubation ways. First method 37℃ shaking 2hr/2hr, Second method RT shaking 3hr/2hr, Third method 1hr/1hr shaking at 37℃. Fourth method RT shaking 3hr method which is the original method of Company C's Kit. Fifth method, the incubation time was shortened under room temperature shaking 2hr, Sixth method 37℃ shaking 2hr. And we performed and compared the correlation and coefficient of each methods.
Results : As a result of performing shortening method on company B currently in use, when comparing the Original method of company B kit, First method 37℃ shaking 2hr/2hr was less than Tg 1.0 ng/mL and the ratio of R²=0.5906, above 1.0 ng/mL In the value, R²=0.9597. Second method RT shaking 3hr/2hr was R²=0.7262 less than value of 1.0 ng/mL, R²=0.9566 above than value of 1.0 ng/mL. Third method 37℃ shaking 1hr/1hr was R²=0.7728 less than value of 1.0 ng/mL, R²=0.8904 above than value of 1.0 ng/mL. Forth, Company C’s The original method, RT shaking 3hr was R²=0.7542 less than value of 1.0 ng/mL, and R²=0.9711 above than value of 1.0 ng/mL. Fifth method RT shaking 2hr was R²=0.5477 less than value of 1.0 ng/mL, R²=0.9231 above than value of 1.0 ng/mL. Sixth method 37℃ shaking 2hr showed R²=0.2848 less than value of 1.0 ng/mL, R²=0.9028 above than value of 1.0 ng/mL.
Conclusion : Samples with both values of 1.0 ng/mL or higher in both of the six methods showed relatively high correlation, but the correlation was relatively low less than value of 1.0 ng/mL. Especially, the 37℃ shaking 2hr method of
company C showed a sharp fluctuation from the low concentration value of 1.0 ng/mL or less. Therefore, we are planning to continuously test the time, equipment, incubation temperature and so on for the room temperature shaking 2hr method and 37℃ shaking 1hr/1hr of company C which showed a relatively high correlation. After that, we can search for an appropriate shortening method through additional experiments such as recovery test, dilution test, sensitivity test, and rovide more accurate and prompt results to the department of medical treatment, It is competitive with EIA test.
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KEYWORD
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Thyroglobulin, Overnight methods, Correlation, Shorten methods
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